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Parameter Set Anti-HIV Drugs - LC-MS/MS

Order No.: 92924/RUO
Parameters:
Amprenavir, Atazanavir, Darunavir, Efavirenz, Elvitegravir, Indinavir, Lopinavir, Maraviroc, Nelfinavir, Nelfinavir-M8, Raltegravir, Ritonavir, Saquinavir

Encompasses 17 analytes
6PLUS1® Multilevel Calibrator Set available
Part of the modular system MassTox® Series A

main product photo

Amprenavir

Atazanavir

Darunavir

Delavirdine

Efavirenz

Elvitegravir

Etravirine

Indinavir

Lopinavir

Maraviroc

Nelfinavir

Nelfinavir-M8

Nevirapine

Raltegravir

Rilpivirine

Ritonavir

Saquinavir

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set Anti-HIV Drugs allows for the fast quantitative determination of amprenavir, atazanavir, darunavir, delavirdine, efavirenz, elvitegravir, etravirine, indinavir, lopinavir, maraviroc, nelfinavir, nelfinavir-M8, nevirapine, raltegravir, rilpivirine, ritonavir and saquinavir in serum/plasma using LC-MS/MS. The use of stable isotopically marked, co-eluating internal standards and the 6PLUS1® multilevel calibrator set ensures high performance of the analysis method.

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Specimen Serum/Plasma
Sample Preparation
  • Reconstitute the Internal Standard Mix.
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A.
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 μl Extraction Buffer.
  • Mix and incubate 2 min at ambient temperature (do not centrifuge).
  • Add 250 μl of mixture A and mix 30 s minimum (vortex).
  • Centrifuge 5 min at 15000 x g.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity.
Run Time ≤ 3.5 min
Injection Volume 0.2 – 50 µl
Gradient

Group 1
0.00–0.20 min       0 % Mobile Phase 2
0.21–2.00 min     70 % Mobile Phase 2
2.01–2.90 min   100 % Mobile Phase 2
2.91–3.50 min       0 % Mobile Phase 2
Group 2
0.00–0.20 min       0 % Mobile Phase 2
0.21–0.60 min     50 % Mobile Phase 2
0.61–2.40 min     90 % Mobile Phase 2
2.41–3.00 min       0 % Mobile Phase 2
Group 3
0.00–0.20 min       0 % Mobile Phase 2
0.21–2.30 min     90 % Mobile Phase 2
2.31–3.10 min       0 % Mobile Phase 2
Ionisation ESI positive
MS/MS Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters Amprenavir, Atazanavir, Darunavir, Efavirenz, Elvitegravir, Indinavir, Lopinavir, Maraviroc, Nelfinavir, Nelfinavir-M8, Raltegravir, Ritonavir, Saquinavir
The following components are included in the kit:
  1. 92053 LCMS TDM Series A 6PLUS1 calibrator anti-HIV drugs
    6PLUS1® Multilevel Plasma Calibrator Set Anti-HIV Drugs
    Order no.: 92053
    MassTox® Series A Anti-HIV Drugs in Serum/Plasma – LC-MS/MS
    Details
  2. 0261 0262 0263 LCMS TDM Series A controls anti-HIV drugs
    MassCheck® Anti-HIV Drugs Plasma Controls
    Order no.: 0261/0262/0263
    Details
  3. Internal Standard Mix Anti-HIV Drugs
    Internal Standard Mix Anti-HIV Drugs
    Order no.: 92844/RUO
    Component of the Parameter Set Anti-HIV Drugs, available separately
    Details
The following products are not included in the kit but are required for the application of the method:
  1. 92110 TDM Series A Master Column
    MassTox® TDM MasterColumn® A
    Order no.: 92110

    Analytical column for MassTox® Series A - LC-MS/MS

    Details
  2. Tuning Mix Anti-HIV Drugs
    Tuning Mix Anti-HIV Drugs
    Order no.: 92042/RUO

    Tuning Mix for Parameter Set Anti-HIV Drugs

    Details
  3. Basic Kit A for 200 Tests - LC-MS/MS
    Basic Kit A for 200 Tests - LC-MS/MS
    Order no.: 92111/200/RUO

    Part of the modular system MassTox® Series A
    Provides all components required for sample prep and all mobile phases

    Details
  4. Basic Kit A for 1000 Tests - LC-MS/MS
    Basic Kit A for 1000 Tests - LC-MS/MS
    Order no.: 92111/1000/RUO

    Part of the modular system MassTox® Series A
    Provides all components required for sample prep and all mobile phases

    Details
  1. 92053 LCMS TDM Series A 6PLUS1 calibrator anti-HIV drugs
    6PLUS1® Multilevel Plasma Calibrator Set Anti-HIV Drugs
    Order no.: 92053
    MassTox® Series A Anti-HIV Drugs in Serum/Plasma – LC-MS/MS
    Details
  1. 0261 0262 0263 LCMS TDM Series A controls anti-HIV drugs
    MassCheck® Anti-HIV Drugs Plasma Controls
    Order no.: 0261/0262/0263
    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. PEEK-encased Prefilter, 2 µm
    PEEK-encased Prefilter, 2 µm
    Order no.: 15011
    Details
  3. Reaction Vials, transparent, 1.5 ml
    Reaction Vials, transparent, 1.5 ml
    Order no.: 33006
    Transparent
    Details
  4. 15070 15071 Prefilter and Housing
    Stainless Steel Prefilter Housing
    Order no.: 15070
    Details
  5. 15070 15071 Prefilter and Housing
    Stainless Steel Prefilter - 0.5 µm
    Order no.: 15071
    Details

Amprenavir

Atazanavir

Darunavir

Delavirdine

Efavirenz

Elvitegravir

Etravirine

Indinavir

Lopinavir

Maraviroc

Nelfinavir

Nelfinavir-M8

Nevirapine

Raltegravir

Rilpivirine

Ritonavir

Saquinavir

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set Anti-HIV Drugs allows for the fast quantitative determination of amprenavir, atazanavir, darunavir, delavirdine, efavirenz, elvitegravir, etravirine, indinavir, lopinavir, maraviroc, nelfinavir, nelfinavir-M8, nevirapine, raltegravir, rilpivirine, ritonavir and saquinavir in serum/plasma using LC-MS/MS. The use of stable isotopically marked, co-eluating internal standards and the 6PLUS1® multilevel calibrator set ensures high performance of the analysis method.

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Specimen Serum/Plasma
Sample Preparation
  • Reconstitute the Internal Standard Mix.
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A.
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 μl Extraction Buffer.
  • Mix and incubate 2 min at ambient temperature (do not centrifuge).
  • Add 250 μl of mixture A and mix 30 s minimum (vortex).
  • Centrifuge 5 min at 15000 x g.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity.
Run Time ≤ 3.5 min
Injection Volume 0.2 – 50 µl
Gradient

Group 1
0.00–0.20 min       0 % Mobile Phase 2
0.21–2.00 min     70 % Mobile Phase 2
2.01–2.90 min   100 % Mobile Phase 2
2.91–3.50 min       0 % Mobile Phase 2
Group 2
0.00–0.20 min       0 % Mobile Phase 2
0.21–0.60 min     50 % Mobile Phase 2
0.61–2.40 min     90 % Mobile Phase 2
2.41–3.00 min       0 % Mobile Phase 2
Group 3
0.00–0.20 min       0 % Mobile Phase 2
0.21–2.30 min     90 % Mobile Phase 2
2.31–3.10 min       0 % Mobile Phase 2
Ionisation ESI positive
MS/MS Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters Amprenavir, Atazanavir, Darunavir, Efavirenz, Elvitegravir, Indinavir, Lopinavir, Maraviroc, Nelfinavir, Nelfinavir-M8, Raltegravir, Ritonavir, Saquinavir
The following components are included in the kit:
  1. 92053 LCMS TDM Series A 6PLUS1 calibrator anti-HIV drugs
    6PLUS1® Multilevel Plasma Calibrator Set Anti-HIV Drugs
    Order no.: 92053
    MassTox® Series A Anti-HIV Drugs in Serum/Plasma – LC-MS/MS
    Details
  2. 0261 0262 0263 LCMS TDM Series A controls anti-HIV drugs
    MassCheck® Anti-HIV Drugs Plasma Controls
    Order no.: 0261/0262/0263
    Details
  3. Internal Standard Mix Anti-HIV Drugs
    Internal Standard Mix Anti-HIV Drugs
    Order no.: 92844/RUO
    Component of the Parameter Set Anti-HIV Drugs, available separately
    Details
The following products are not included in the kit but are required for the application of the method:
  1. 92110 TDM Series A Master Column
    MassTox® TDM MasterColumn® A
    Order no.: 92110

    Analytical column for MassTox® Series A - LC-MS/MS

    Details
  2. Tuning Mix Anti-HIV Drugs
    Tuning Mix Anti-HIV Drugs
    Order no.: 92042/RUO

    Tuning Mix for Parameter Set Anti-HIV Drugs

    Details
  3. Basic Kit A for 200 Tests - LC-MS/MS
    Basic Kit A for 200 Tests - LC-MS/MS
    Order no.: 92111/200/RUO

    Part of the modular system MassTox® Series A
    Provides all components required for sample prep and all mobile phases

    Details
  4. Basic Kit A for 1000 Tests - LC-MS/MS
    Basic Kit A for 1000 Tests - LC-MS/MS
    Order no.: 92111/1000/RUO

    Part of the modular system MassTox® Series A
    Provides all components required for sample prep and all mobile phases

    Details
  1. 92053 LCMS TDM Series A 6PLUS1 calibrator anti-HIV drugs
    6PLUS1® Multilevel Plasma Calibrator Set Anti-HIV Drugs
    Order no.: 92053
    MassTox® Series A Anti-HIV Drugs in Serum/Plasma – LC-MS/MS
    Details
  1. 0261 0262 0263 LCMS TDM Series A controls anti-HIV drugs
    MassCheck® Anti-HIV Drugs Plasma Controls
    Order no.: 0261/0262/0263
    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. PEEK-encased Prefilter, 2 µm
    PEEK-encased Prefilter, 2 µm
    Order no.: 15011
    Details
  3. Reaction Vials, transparent, 1.5 ml
    Reaction Vials, transparent, 1.5 ml
    Order no.: 33006
    Transparent
    Details
  4. 15070 15071 Prefilter and Housing
    Stainless Steel Prefilter Housing
    Order no.: 15070
    Details
  5. 15070 15071 Prefilter and Housing
    Stainless Steel Prefilter - 0.5 µm
    Order no.: 15071
    Details