Parameter Set Antiepileptic Drugs - LC-MS/MS

Order No.: 92921/RUO

Parameters:

10-OH-Carbamazepine, Carbamazepine, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, N-Desmethylmesuximide, Phenobarbital, Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide

Encompasses 26 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the modular system MassTox® Series A

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Chromatogram

Parameters

Description

Technical Data

Kit Components

Required Components

Calibrators / Controls

Lab Equipment

Carbamazepine

Carbamazepine-10,11-epoxide

10,11-Dihydroxycarbamazepine

10-OH-Carbamazepine

Ethosuximide

Felbamate

Gabapentin

Lacosamide

Lamotrigine

Levetiracetam (Keppra®)

N-Desmethylmesuximide

Oxcarbazepine

Phenobarbital

Phenylethylmalonamide (PEMA)

Phenytoin

Pregabalin

Primidone

Rufinamide

Stiripentol

Sultiame

Theophyllie

Tiagabine

Topiramate

Valproic acid

Vigabatrin

Zonisamid

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Antiepileptic Drugs allows for the fast quantitative determination of 26 different analytes in serum/plasma by LC-MS/MS. The use of stable isotopically marked and co-eluating internal standards ensures high performance of the analysis method.

For the analysis you require the MassTox® Basic Kit A, the specifc MassTox® Parameter Set and the analytical column MassTox® MasterColumn® A.

More Information
Method of Analysis	LC-MS/MS
Please note	The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Specimen	Serum/Plasma
Sample Preparation	Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A Pipette 50 µl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min Dilute supernatant with Dilution Buffer prior to injection, depending on instrument sensitivity.
Run Time	2.5 – 3.5 min
Injection Volume	0.2 – 50 µl
Gradient	Starting point: 0% Mobile Phase 2 Group 1 0.00→2.00 min, 100 % Mobile Phase 2 2.00-2.60 min, 100 % Mobile Phase 2 2.61-3.50 min, 0 % Mobile Phase 2 Group 2 and 3 0.00→1.00 min, 100 % Mobile Phase 2 1.00-1.60 min, 100 % Mobile Phase 2 1.61-2.50 min, 0 % Mobile Phase 2 Group 4 and 5 0.00→1.00 min, 100 % Mobile Phase 2 1.00-1.80 min, 100 % Mobile Phase 2 1.81-2.70 min, 0 % Mobile Phase 2
Ionisation	ESI positive and negative
MS/MS Mode	MRM
Additional Info	We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters	10-OH-Carbamazepine, Carbamazepine, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, N-Desmethylmesuximide, Phenobarbital, Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide

The following components are included in the kit:

3PLUS1® Multilevel Plasma Calibrator Set Antiepileptic Drugs/EXTENDED
Order no.: 92025/XT

MassTox® Series A Antiepileptic Drugs in Serum/Plasma – LC-MS/MS

Details
MassCheck® Antiepileptic Drugs/EXTENDED Plasma Controls
Order no.: 0249/XT; 0250/XT; 0251/XT

MassTox® Series A Antiepileptic Drugs in Serum/Plasma – LC-MS/MS

Details
Internal Standard Mix Antiepileptic Drugs
Order no.: 92546/RUO

Component of the Parameter Set Antiepileptic Drugs, available separately

Details

The following products are not included in the kit but are required for the application of the method:

MassTox® TDM MasterColumn® A
Order no.: 92110

Analytical column for MassTox® Series A - LC-MS/MS

Details
Tuning Mix Antiepileptic Drugs/EXTENDED 1
Order no.: 92034/XT/RUO

Tuning Mix 1 for the Parameter Set Antiepileptic Drugs - LC-MS/MS

Details
Tuning Mix Antiepileptic Drugs/EXTENDED 2
Order no.: 92035/XT/RUO

Tuning Mix 2 for the Parameter Set Antiepileptic Drugs - LC-MS/MS

Details
Tuning Mix Antiepileptic Drugs/EXTENDED 3
Order no.: 92036/XT/RUO

Tuning Mix 3 for the Parameter Set Antiepileptic Drugs - LC-MS/MS

Details
Tuning Mix Antiepileptic Drugs/EXTENDED 4
Order no.: 92037/XT/RUO

Tuning Mix 4 for the Parameter Set Antiepileptic Drugs - LC-MS/MS

Details
Tuning Mix Antiepileptic Drugs/EXTENDED 5
Order no.: 92038/XT/RUO

Tuning Mix 5 for the Parameter Set Antiepileptic Drugs - LC-MS/MS

Details
Basic Kit A for 200 Tests - LC-MS/MS
Order no.: 92111/200/RUO

Part of the modular system MassTox® Series A
Provides all components required for sample prep and all mobile phases

Details
Basic Kit A for 1000 Tests - LC-MS/MS
Order no.: 92111/1000/RUO

Part of the modular system MassTox® Series A
Provides all components required for sample prep and all mobile phases

Details

3PLUS1® Multilevel Plasma Calibrator Set Antiepileptic Drugs/EXTENDED
Order no.: 92025/XT

MassTox® Series A Antiepileptic Drugs in Serum/Plasma – LC-MS/MS

Details

MassCheck® Antiepileptic Drugs/EXTENDED Plasma Controls
Order no.: 0249/XT; 0250/XT; 0251/XT

MassTox® Series A Antiepileptic Drugs in Serum/Plasma – LC-MS/MS

Details

PEEK Prefilter Housing
Order no.: 15010

Details
PEEK-encased Prefilter, 2 µm
Order no.: 15011

Details
Reaction Vials, transparent, 1.5 ml
Order no.: 33006

Transparent

Details
Stainless Steel Prefilter Housing
Order no.: 15070

Details
Stainless Steel Prefilter - 0.5 µm
Order no.: 15071

Details