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Parameter Set Mycophenolic Acid - LC-MS/MS

Order No.: 92916/RUO
Parameters:
Mycophenolic Acid, Mycophenolic Acid Glucuronide

For the analysis of MPA and MPAG
6PLUS1® Multilevel Calibrator Set available
Part of the modular system MassTox® Series A

main product photo

Mycophenolic Acid

Mycophenolic Acid Glucuronide

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Mycophenolic Acid allows for the fast quantitative determination of mycophenolic acid and its glucuronide in serum/plasma by LC-MS/MS. The use of stable isotopically marked, co-eluating internal standards and the 6PLUS1® multilevel calibrators ensures high performance of the analysis method.

For the analysis you require the MassTox® Basic Kit A, the specifc MassTox® Parameter Set and the analytical column MassTox® MasterColumn® A.

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Specimen Serum/Plasma
Sample Preparation
  • Reconstitute the Internal Standard Mix.
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity
Run Time 1.8 min
Injection Volume 0.2 – 50 µl
Gradient

Starting point       60 % Mobile Phase 2
0.00→0.50 min    100 % Mobile Phase 2
0.51–1.00 min    100 % Mobile Phase 2
1.01→1.10 min     60 % Mobile Phase 2
1.11–1.81 min      60 % Mobile Phase 2
Ionisation ESI positive
MS/MS Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters Mycophenolic Acid, Mycophenolic Acid Glucuronide
The following components are included in the kit:
  1. 46039XL 46039 LCMS TDM Series A 6PLUS1 calibrator mycophenolic acid
    6PLUS1® Multilevel Plasma Calibrator Set Mycophenolic Acid/Glucuronide
    Order no.: 46039/46039XL
    MassTox® Series A Mycophenolic Acid in Serum/Plasma – LC-MS/MS
    Details
  2. 0234 0235 0236 LCMS TDM Series A controls mycophenolic acid
    MassCheck® Mycophenolic Acid/Glucuronide Plasma Controls
    Order no.: 0234/0235/0236

    MassTox® Series A in Serum/Plasma – LC-MS/MS

    Details
  3. Internal Standard Set Mycophenolic Acid/Glucuronide
    Internal Standard Set Mycophenolic Acid/Glucuronide
    Order no.: 92246/RUO
    Component of the Parameter Set Mycophenolic Acid/Glucuronide, available separately
    Details
The following products are not included in the kit but are required for the application of the method:
  1. 92110 TDM Series A Master Column
    MassTox® TDM MasterColumn® A
    Order no.: 92110

    Analytical column for MassTox® Series A - LC-MS/MS

    Details
  2. Tuning Mix Mycophenolic Acid/Glucuronide
    Tuning Mix Mycophenolic Acid/Glucuronide
    Order no.: 92019/RUO
    Tuning Mix for the Parameter Set Mycophenolic Acid/Glucuronide - LC-MS/MS
    Details
  3. Basic Kit A for 200 Tests - LC-MS/MS
    Basic Kit A for 200 Tests - LC-MS/MS
    Order no.: 92111/200/RUO

    Part of the modular system MassTox® Series A
    Provides all components required for sample prep and all mobile phases

    Details
  4. Basic Kit A for 1000 Tests - LC-MS/MS
    Basic Kit A for 1000 Tests - LC-MS/MS
    Order no.: 92111/1000/RUO

    Part of the modular system MassTox® Series A
    Provides all components required for sample prep and all mobile phases

    Details
  1. 46039XL 46039 LCMS TDM Series A 6PLUS1 calibrator mycophenolic acid
    6PLUS1® Multilevel Plasma Calibrator Set Mycophenolic Acid/Glucuronide
    Order no.: 46039/46039XL
    MassTox® Series A Mycophenolic Acid in Serum/Plasma – LC-MS/MS
    Details
  2. 46029 LCMS TDM Series A 3PLUS1 calibrator mycophenolic acid
    3PLUS1® Multilevel Plasma Calibrator Set Mycophenolic Acid/Glucuronide
    Order no.: 46029
    MassTox® Series A Mycophenolic Acid in Serum/Plasma – LC-MS/MS
    Details
  1. 0234 0235 0236 LCMS TDM Series A controls mycophenolic acid
    MassCheck® Mycophenolic Acid/Glucuronide Plasma Controls
    Order no.: 0234/0235/0236

    MassTox® Series A in Serum/Plasma – LC-MS/MS

    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. PEEK-encased Prefilter, 2 µm
    PEEK-encased Prefilter, 2 µm
    Order no.: 15011
    Details
  3. Reaction Vials, transparent, 1.5 ml
    Reaction Vials, transparent, 1.5 ml
    Order no.: 33006
    Transparent
    Details
  4. 15070 15071 Prefilter and Housing
    Stainless Steel Prefilter Housing
    Order no.: 15070
    Details
  5. 15070 15071 Prefilter and Housing
    Stainless Steel Prefilter - 0.5 µm
    Order no.: 15071
    Details

Mycophenolic Acid

Mycophenolic Acid Glucuronide

The modular system MassTox® Basic Kit A in combination with the MassTox® Parameter Set for Mycophenolic Acid allows for the fast quantitative determination of mycophenolic acid and its glucuronide in serum/plasma by LC-MS/MS. The use of stable isotopically marked, co-eluating internal standards and the 6PLUS1® multilevel calibrators ensures high performance of the analysis method.

For the analysis you require the MassTox® Basic Kit A, the specifc MassTox® Parameter Set and the analytical column MassTox® MasterColumn® A.

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Specimen Serum/Plasma
Sample Preparation
  • Reconstitute the Internal Standard Mix.
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl research sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity
Run Time 1.8 min
Injection Volume 0.2 – 50 µl
Gradient

Starting point       60 % Mobile Phase 2
0.00→0.50 min    100 % Mobile Phase 2
0.51–1.00 min    100 % Mobile Phase 2
1.01→1.10 min     60 % Mobile Phase 2
1.11–1.81 min      60 % Mobile Phase 2
Ionisation ESI positive
MS/MS Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters Mycophenolic Acid, Mycophenolic Acid Glucuronide
The following components are included in the kit:
  1. 46039XL 46039 LCMS TDM Series A 6PLUS1 calibrator mycophenolic acid
    6PLUS1® Multilevel Plasma Calibrator Set Mycophenolic Acid/Glucuronide
    Order no.: 46039/46039XL
    MassTox® Series A Mycophenolic Acid in Serum/Plasma – LC-MS/MS
    Details
  2. 0234 0235 0236 LCMS TDM Series A controls mycophenolic acid
    MassCheck® Mycophenolic Acid/Glucuronide Plasma Controls
    Order no.: 0234/0235/0236

    MassTox® Series A in Serum/Plasma – LC-MS/MS

    Details
  3. Internal Standard Set Mycophenolic Acid/Glucuronide
    Internal Standard Set Mycophenolic Acid/Glucuronide
    Order no.: 92246/RUO
    Component of the Parameter Set Mycophenolic Acid/Glucuronide, available separately
    Details
The following products are not included in the kit but are required for the application of the method:
  1. 92110 TDM Series A Master Column
    MassTox® TDM MasterColumn® A
    Order no.: 92110

    Analytical column for MassTox® Series A - LC-MS/MS

    Details
  2. Tuning Mix Mycophenolic Acid/Glucuronide
    Tuning Mix Mycophenolic Acid/Glucuronide
    Order no.: 92019/RUO
    Tuning Mix for the Parameter Set Mycophenolic Acid/Glucuronide - LC-MS/MS
    Details
  3. Basic Kit A for 200 Tests - LC-MS/MS
    Basic Kit A for 200 Tests - LC-MS/MS
    Order no.: 92111/200/RUO

    Part of the modular system MassTox® Series A
    Provides all components required for sample prep and all mobile phases

    Details
  4. Basic Kit A for 1000 Tests - LC-MS/MS
    Basic Kit A for 1000 Tests - LC-MS/MS
    Order no.: 92111/1000/RUO

    Part of the modular system MassTox® Series A
    Provides all components required for sample prep and all mobile phases

    Details
  1. 46039XL 46039 LCMS TDM Series A 6PLUS1 calibrator mycophenolic acid
    6PLUS1® Multilevel Plasma Calibrator Set Mycophenolic Acid/Glucuronide
    Order no.: 46039/46039XL
    MassTox® Series A Mycophenolic Acid in Serum/Plasma – LC-MS/MS
    Details
  2. 46029 LCMS TDM Series A 3PLUS1 calibrator mycophenolic acid
    3PLUS1® Multilevel Plasma Calibrator Set Mycophenolic Acid/Glucuronide
    Order no.: 46029
    MassTox® Series A Mycophenolic Acid in Serum/Plasma – LC-MS/MS
    Details
  1. 0234 0235 0236 LCMS TDM Series A controls mycophenolic acid
    MassCheck® Mycophenolic Acid/Glucuronide Plasma Controls
    Order no.: 0234/0235/0236

    MassTox® Series A in Serum/Plasma – LC-MS/MS

    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. PEEK-encased Prefilter, 2 µm
    PEEK-encased Prefilter, 2 µm
    Order no.: 15011
    Details
  3. Reaction Vials, transparent, 1.5 ml
    Reaction Vials, transparent, 1.5 ml
    Order no.: 33006
    Transparent
    Details
  4. 15070 15071 Prefilter and Housing
    Stainless Steel Prefilter Housing
    Order no.: 15070
    Details
  5. 15070 15071 Prefilter and Housing
    Stainless Steel Prefilter - 0.5 µm
    Order no.: 15071
    Details