Vitamins A and E in Serum/Plasma - HPLC Basic Kit
Short run time of less than 3 min
Easy sample preparation
High eluate stability
Assay validated according to IVDR (=> Declaration of Conformity)
Vitamin A
Vitamin E
Clinical relevance
This assay allows the quantitative determination of uroporphyrin, heptacarboxyporphyrin, hexacarboxyporphyrin, pentacarboxyporphyrin, coproporphyrin I and coproporphyrin III in human urine samples.
It is intended to be used for patients in whom the urinary levels of uroporphyrin, heptacarboxyporphyrin, hexacarboxyporphyrin, pentacarboxyporphyrin, coproporphyrin I and coproporphyrin III are of clinical importance, primarily as an aid to diagnosis of suspected acute intermittent porphyria, porphyria variegata, aminolevulinic acid dehydratase deficiency porphyria, hereditary coproporphyria, porphyria cutanea tarda or congenital erythropoietic porphyria and/or for their differential diagnosis.
Product advantages
• Easy sample preparation
• Stable standards and controls
Assay characteristics
Sample preparation consists of sample stabilisation, addition of the internal standard followed by centrifugation to separate particles that may be present in the urine. An oxidation step is not necessary as the porphyrinogens oxidise spontaneously by air into the fluorescent porphyrins. The internal standard elutes between the analytes thus avoiding analytical variation and inaccuracy as well as allowing a shortening of the chromatographic run time.
Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual.
Two types of kits available
Basic Kit
• Only sample preparation reagents and vials included
• No excess quantities of mobile phase and calibrators
|
34400-BK Basic Kit |
|
| 34404 | Internal Standard, 40 ml (1x) |
| 34405 | Precipitation Reagent, 40 ml (1x) |
| 33005 | Reaction Vials, 1.5 ml, amber coloured (light protection), 100 pcs. (2x) |
Reagent Kit
• Mobile phase and calibrator included
|
34400 Reagent Kit |
|
| 34001 | Mobile Phase, 1000 ml (1x) |
| 34004 | Serum Calibration Standard, 5 x 1 ml (lyoph.) (1x) |
| 34404 | Internal Standard, 40 ml (1x) |
| 34405 | Precipitation Reagent, 40 ml (1x) |
| 33005 | Reaction Vials, 1.5 ml, amber coloured (light protection), 100 pcs. (2x) |
| Method of Analysis | HPLC |
|---|---|
| Sample Preparations | 200 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Uroporphyrin: 0.48 µg/l – 1400 µg/l Different systems might show different performance data. |
| Specimen | Urine |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 20 min |
| Injection Volume | 25 µl |
| Flow Rate | 1.2 ml/min |
| Column Temperature | +20 to +25 °C |
| Gradient | binary |
| Wavelength | EX 405 nm EM 620 nm |
| Additional Info | Any binary HPLC gradient system with fluorescence detector is suitable. |
| Parameters | Vitamin A, Vitamin E |
Vitamins A and E in Serum/Plasma - HPLC Basic Kit
For 200 sample preparations
| Order No. | Basic Kit components (Quantities in kit) |
| 34404 | Internal Standard, 40 ml (1x) |
| 34405 | Precipitation Reagent, 40 ml (1x) |
| 33005 | Reaction Vials, 1.5 ml, amber coloured (light protection), 100 pcs. (1x) |
| Order No. | Required components (not included in the Basic Kit) |
| 34300 | HPLC Column, equilibrated, with test chromatogram, 1 pc. |
| 34001 | Mobile Phase, 1000 ml |
| 34004 | Serum Calibration Standard, 5 x 1 ml (lyoph.) |
| 0036 | Serum Control, Level I, 5 x 2 ml (lyoph.) |
| 0037 | Serum Control, Level II, 5 x 2 ml (lyoph.) |
| Order No. | General lab equipment (non-CE/IVD products) |
| 33005 | Reaction Vials, 1.5 ml, amber coloured (light protection), 100 pcs. |
| 15070 | Stainless Steel Prefilter Housing, 1 pc. |
| 15012 | PEEK-encased Prefilter, 0.5 µm,1 pc. |
-
Vitamins A and E Serum Calibration StandardOrder no.: 34004Vitamins A and E in Serum/Plasma- HPLC/UHPLC
Vitamin A
Vitamin E
Clinical relevance
This assay allows the quantitative determination of uroporphyrin, heptacarboxyporphyrin, hexacarboxyporphyrin, pentacarboxyporphyrin, coproporphyrin I and coproporphyrin III in human urine samples.
It is intended to be used for patients in whom the urinary levels of uroporphyrin, heptacarboxyporphyrin, hexacarboxyporphyrin, pentacarboxyporphyrin, coproporphyrin I and coproporphyrin III are of clinical importance, primarily as an aid to diagnosis of suspected acute intermittent porphyria, porphyria variegata, aminolevulinic acid dehydratase deficiency porphyria, hereditary coproporphyria, porphyria cutanea tarda or congenital erythropoietic porphyria and/or for their differential diagnosis.
Product advantages
• Easy sample preparation
• Stable standards and controls
Assay characteristics
Sample preparation consists of sample stabilisation, addition of the internal standard followed by centrifugation to separate particles that may be present in the urine. An oxidation step is not necessary as the porphyrinogens oxidise spontaneously by air into the fluorescent porphyrins. The internal standard elutes between the analytes thus avoiding analytical variation and inaccuracy as well as allowing a shortening of the chromatographic run time.
Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual.
Two types of kits available
Basic Kit
• Only sample preparation reagents and vials included
• No excess quantities of mobile phase and calibrators
|
34400-BK Basic Kit |
|
| 34404 | Internal Standard, 40 ml (1x) |
| 34405 | Precipitation Reagent, 40 ml (1x) |
| 33005 | Reaction Vials, 1.5 ml, amber coloured (light protection), 100 pcs. (2x) |
Reagent Kit
• Mobile phase and calibrator included
|
34400 Reagent Kit |
|
| 34001 | Mobile Phase, 1000 ml (1x) |
| 34004 | Serum Calibration Standard, 5 x 1 ml (lyoph.) (1x) |
| 34404 | Internal Standard, 40 ml (1x) |
| 34405 | Precipitation Reagent, 40 ml (1x) |
| 33005 | Reaction Vials, 1.5 ml, amber coloured (light protection), 100 pcs. (2x) |
| Method of Analysis | HPLC |
|---|---|
| Sample Preparations | 200 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Uroporphyrin: 0.48 µg/l – 1400 µg/l Different systems might show different performance data. |
| Specimen | Urine |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 20 min |
| Injection Volume | 25 µl |
| Flow Rate | 1.2 ml/min |
| Column Temperature | +20 to +25 °C |
| Gradient | binary |
| Wavelength | EX 405 nm EM 620 nm |
| Additional Info | Any binary HPLC gradient system with fluorescence detector is suitable. |
| Parameters | Vitamin A, Vitamin E |
Vitamins A and E in Serum/Plasma - HPLC Basic Kit
For 200 sample preparations
| Order No. | Basic Kit components (Quantities in kit) |
| 34404 | Internal Standard, 40 ml (1x) |
| 34405 | Precipitation Reagent, 40 ml (1x) |
| 33005 | Reaction Vials, 1.5 ml, amber coloured (light protection), 100 pcs. (1x) |
| Order No. | Required components (not included in the Basic Kit) |
| 34300 | HPLC Column, equilibrated, with test chromatogram, 1 pc. |
| 34001 | Mobile Phase, 1000 ml |
| 34004 | Serum Calibration Standard, 5 x 1 ml (lyoph.) |
| 0036 | Serum Control, Level I, 5 x 2 ml (lyoph.) |
| 0037 | Serum Control, Level II, 5 x 2 ml (lyoph.) |
| Order No. | General lab equipment (non-CE/IVD products) |
| 33005 | Reaction Vials, 1.5 ml, amber coloured (light protection), 100 pcs. |
| 15070 | Stainless Steel Prefilter Housing, 1 pc. |
| 15012 | PEEK-encased Prefilter, 0.5 µm,1 pc. |
-
Vitamins A and E Serum Calibration StandardOrder no.: 34004Vitamins A and E in Serum/Plasma- HPLC/UHPLC