Parameter Set Mycophenolic Acid - LC-MS/MS
For the analysis of MPA and MPAG
3PLUS1® and 6PLUS1® Multilevel Calibrator Sets available
Part of the continuously updated MassTox® TDM Series A
Sample preparation with 96 well filter plates also possible
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
Mycophenolic Acid
Mycophenolic Acid Glucuronide
Clinical relevance
Mycophenolic acid (MPA) is used as an immunosuppressant agent to prevent organ transplant rejection. MPA selectively inhibits the synthesis of purines and specifically reduces the growth of B- and T-lymphocytes. Furthermore, MPA is also used to treat autoimmune diseases such as psoriasis, systemic lupus erythematosus or scleroderma.
Therapeutic monitoring of MPA in the blood is required to adjust individual concentrations within the therapeutic window and to attain a favourable ratio between therapeutic effects and side effects. Currently available drugs contain mycophenolate mofetil or mycophenolate sodium, which are both completely metabolised into the active metabolite MPA. For excretion, MPA is bound to glucuronic acid.
This parameter set allows the quantitative determination of mycophenolic acid and its metabolite mycophenolic acid glucuronide in human serum or plasma samples by LC-MS/MS (liquid chromatography mass spectrometry).
It is intended as a monitoring test for patients treated with mycophenolate mofetil or mycophenolate sodium as an aid to ensure drug levels are maintained within the therapeutic range.
MassTox® TDM Serie A
The Parameter Set is a part of the Series A modular system, which enables the analysis of all parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory.
It consists of:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A
More information about MassTox® TDM Series A
MassSTAR
For this parameter set a CE-IVD compliant workflow method is also available using the MassSTAR to automate the sample preparation.
More information about MassSTAR
| Method of Analysis | LC-MS/MS |
|---|---|
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | MPA: 0.2 – 75 mg/l Different systems might show different performance data. |
| Specimen | Serum/Plasma |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 1.8 min |
| Injection Volume | 0.2 – 50 µl (mass spectrometer dependent) |
| Gradient | binary |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | Mycophenolic Acid, Mycophenolic Acid Glucuronide |
-
Internal Standard Set Mycophenolic Acid/GlucuronideOrder no.: 92246Component of the Parameter Set Mycophenolic Acid/Glucuronide, available separately -
3PLUS1® Multilevel Plasma Calibrator Set Mycophenolic Acid/GlucuronideOrder no.: 46029MassTox® TDM Series A Mycophenolic Acid in Serum/Plasma – LC-MS/MS -
MassCheck® Mycophenolic Acid/Glucuronide Plasma ControlsOrder no.: 0234/0235/0236MassTox® TDM Series A in Serum/Plasma – LC-MS/MS
-
MassTox® TDM MasterColumn® AOrder no.: 92110Analytical column for MassTox® TDM Series A - LC-MS/MS
-
Tuning Mix Mycophenolic Acid/GlucuronideOrder no.: 92019Tuning Mix for the Parameter Set Mycophenolic Acid/Glucuronide - LC-MS/MS -
Basic Kit A for 200 Tests - LC-MS/MSOrder no.: 92111/200Part of the MassTox® TDM Series A
Modular system for therapeutic drug monitoring
Provides all components required for sample prep and all mobile phasesCE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
-
Basic Kit A for 1000 Tests - LC-MS/MSOrder no.: 92111/1000Part of the MassTox® TDM Series A
Modular system for therapeutic drug monitoring
Provides all components required for sample prep and all mobile phasesCE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
-
Basic Kit A for 1000 Tests with 96 Well Filter Plates - LC-MS/MSOrder no.: 92111/1000/FPart of the MassTox® TDM Series A
Modular system for therapeutic drug monitoring
Provides all components required for sample prep and all mobile phases
Sample preparation with 96 Well Filter PlatesCE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
-
3PLUS1® Multilevel Plasma Calibrator Set Mycophenolic Acid/GlucuronideOrder no.: 46029MassTox® TDM Series A Mycophenolic Acid in Serum/Plasma – LC-MS/MS
-
6PLUS1® Multilevel Plasma Calibrator Set Mycophenolic Acid/GlucuronideOrder no.: 46039/46039XLMassTox® TDM Series A Mycophenolic Acid in Serum/Plasma – LC-MS/MS
-
MassCheck® Mycophenolic Acid/Glucuronide Plasma ControlsOrder no.: 0234/0235/0236
MassTox® TDM Series A in Serum/Plasma – LC-MS/MS
Mycophenolic Acid
Mycophenolic Acid Glucuronide
Clinical relevance
Mycophenolic acid (MPA) is used as an immunosuppressant agent to prevent organ transplant rejection. MPA selectively inhibits the synthesis of purines and specifically reduces the growth of B- and T-lymphocytes. Furthermore, MPA is also used to treat autoimmune diseases such as psoriasis, systemic lupus erythematosus or scleroderma.
Therapeutic monitoring of MPA in the blood is required to adjust individual concentrations within the therapeutic window and to attain a favourable ratio between therapeutic effects and side effects. Currently available drugs contain mycophenolate mofetil or mycophenolate sodium, which are both completely metabolised into the active metabolite MPA. For excretion, MPA is bound to glucuronic acid.
This parameter set allows the quantitative determination of mycophenolic acid and its metabolite mycophenolic acid glucuronide in human serum or plasma samples by LC-MS/MS (liquid chromatography mass spectrometry).
It is intended as a monitoring test for patients treated with mycophenolate mofetil or mycophenolate sodium as an aid to ensure drug levels are maintained within the therapeutic range.
MassTox® TDM Serie A
The Parameter Set is a part of the Series A modular system, which enables the analysis of all parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory.
It consists of:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A
More information about MassTox® TDM Series A
MassSTAR
For this parameter set a CE-IVD compliant workflow method is also available using the MassSTAR to automate the sample preparation.
More information about MassSTAR
| Method of Analysis | LC-MS/MS |
|---|---|
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | MPA: 0.2 – 75 mg/l Different systems might show different performance data. |
| Specimen | Serum/Plasma |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 1.8 min |
| Injection Volume | 0.2 – 50 µl (mass spectrometer dependent) |
| Gradient | binary |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | Mycophenolic Acid, Mycophenolic Acid Glucuronide |
-
Internal Standard Set Mycophenolic Acid/GlucuronideOrder no.: 92246Component of the Parameter Set Mycophenolic Acid/Glucuronide, available separately
-
3PLUS1® Multilevel Plasma Calibrator Set Mycophenolic Acid/GlucuronideOrder no.: 46029MassTox® TDM Series A Mycophenolic Acid in Serum/Plasma – LC-MS/MS
-
MassCheck® Mycophenolic Acid/Glucuronide Plasma ControlsOrder no.: 0234/0235/0236
MassTox® TDM Series A in Serum/Plasma – LC-MS/MS
-
MassTox® TDM MasterColumn® AOrder no.: 92110
Analytical column for MassTox® TDM Series A - LC-MS/MS
-
Tuning Mix Mycophenolic Acid/GlucuronideOrder no.: 92019Tuning Mix for the Parameter Set Mycophenolic Acid/Glucuronide - LC-MS/MS
-
Basic Kit A for 200 Tests - LC-MS/MSOrder no.: 92111/200
Part of the MassTox® TDM Series A
Modular system for therapeutic drug monitoring
Provides all components required for sample prep and all mobile phasesCE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
-
Basic Kit A for 1000 Tests - LC-MS/MSOrder no.: 92111/1000
Part of the MassTox® TDM Series A
Modular system for therapeutic drug monitoring
Provides all components required for sample prep and all mobile phasesCE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
-
Basic Kit A for 1000 Tests with 96 Well Filter Plates - LC-MS/MSOrder no.: 92111/1000/F
Part of the MassTox® TDM Series A
Modular system for therapeutic drug monitoring
Provides all components required for sample prep and all mobile phases
Sample preparation with 96 Well Filter PlatesCE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
-
3PLUS1® Multilevel Plasma Calibrator Set Mycophenolic Acid/GlucuronideOrder no.: 46029MassTox® TDM Series A Mycophenolic Acid in Serum/Plasma – LC-MS/MS
-
6PLUS1® Multilevel Plasma Calibrator Set Mycophenolic Acid/GlucuronideOrder no.: 46039/46039XLMassTox® TDM Series A Mycophenolic Acid in Serum/Plasma – LC-MS/MS
-
MassCheck® Mycophenolic Acid/Glucuronide Plasma ControlsOrder no.: 0234/0235/0236
MassTox® TDM Series A in Serum/Plasma – LC-MS/MS