Vitamin B1/B6 in Whole Blood, fully automated analysis - UHPLC

Clinical relevance

Vitamin B₁ (thiamine) plays an important role in deriving energy from carbohydrates and fats. The active form is thiamine pyrophosphate (TPP), a coenzyme for some enzymatic reactions in carbohydrate metabolism and for pyruvate decarboxylase. TPP is also significantly involved in the oxidative degradation of glucose. The determination of the TPP concentration is more significant than measurements of total thiamine, as by this method only the physiologically active form is taken into account.

Vitamin B₆ comprises pyridoxine, pyridoxamine and pyridoxal, as well as their phosphate derivatives. It is ingested with food and transferred via several enzymatic conversions into its active form, pyridoxal-5’-phosphate (PLP). PLP is involved in nearly one hundred different enzymatic reactions, among other things, as co-factor in amino acid metabolism, and in the formation of haemoglobin or neurotransmitters in the brain.

Product advantages

• Maximum throughput: Automated sample preparation and fast UHPLC in less than 3 minutes
• Reliable and reproducible results
• Each parameter safeguarded by a specific internal standard

This UHPLC assay facilitates the combined analysis of vitamin B₁ in whole blood and vitamin B₆ in whole blood or plasma. Sample preparation is automated in 96 well filter plates for maximum output and minimal workload. UHPLC requires less than 3 minutes per sample. The internal standard for each of the two parameters and special quality controls ensure precise and reliable quantification.

Chromsystems also offers two assays that feature manual determination using HPLC: a standard version as well as a method for simplified sample preparation with pre-mixed tubes.

Alternatively, an automated method using HPLC is also available.

Content for 960 analyses
Mobile Phase A (for UHPLC), 2 x 1000 ml
Mobile Phase B (for UHPLC), 1000 ml
UHPLC Column, 1 pc.
Vitamins B1/B6 Whole Blood Calibration Standard (lyoph.), 5 x 5 x 1 ml
Internal Standard Mix, 50 ml
Extraction Reagent, 2 x 75 ml
Prep Solution, 3 x 25 ml
Finisher 1 (lyoph.), 2 x 5 x 0.25 ml
Finisher 2 , 50 ml
Dilution Buffer, 100 ml
96 Well Filter Plates, 2 x 5 pcs.
96 Well Collection Plates, 2 x 10 pcs.
Pierceable Adhesive Seals for 96 Well Plates, 2 x 5 pcs.