Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC

Clinical relevance

Vitamin B₁ (thiamine) plays an important role in deriving energy from carbohydrates and fats. The active form is thiamine pyrophosphate (TPP), a coenzyme for some enzymatic reactions in carbohydrate metabolism and for pyruvate decarboxylase. TPP is also significantly involved in the oxidative degradation of glucose. The determination of the TPP concentration is more significant than measurements of total thiamine, as by this method only the physiologically active form is taken into account.

Vitamin B₆ comprises pyridoxine, pyridoxamine and pyridoxal, as well as their phosphate derivatives. It is ingested with food and transferred via several enzymatic conversions into its active form, pyridoxal-5’-phosphate (PLP). PLP is involved in nearly one hundred different enzymatic reactions, among other things, as co-factor in amino acid metabolism, and in the formation of haemoglobin or neurotransmitters in the brain.

Product advantages

• Specific internal standard for each parameter
• No post-column derivatisation required
• One sample preparation and one run for both parameters

This Chromsystems kit facilitates the combined analysis of vitamin B₁ in whole blood and vitamin B₆ in whole blood or plasma. The sample preparation can be processed from different matrices, so that both whole blood samples and plasma samples can easily be measured in the same run. Vitamin molecules are already derivatised during sample preparation, which renders the common post-column derivatisation unnecessary. The separation takes place with a binary gradient. The internal standard for each of the two parameters and special quality controls ensure precise and reliable quantification.

This method is also available with simplified sample preparation in pre-mixed tubes.

There is also a method using UHPLC as well as automated sample preparation for HPLC and UHPLC methods available for laboratories with a higher sample throughput.