Vitamin B1 in Whole Blood - HPLC
No post-column derivatisation
Easy handling
Highly stable calibration standards and controls
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
Vitamin B1 (TPP, thiamine pyrophosphate)
Clinical relevance
Vitamin B1 (thiamine) plays an important role in deriving energy from carbohydrates and fats. The active form is thiamine pyrophosphate (TPP), a coenzyme for some enzymatic reactions in the carbohydrate metabolism and for the pyruvate decarboxylase. TPP is also significantly involved in the oxidative degradation of glucose. The determination of TPP is more significant than measurements of total thiamine, as by this method only the physiologically active form is taken into account.
Thiamine need is closely associated with caloric intake due to its central role in the energy metabolism: the more energy is needed, e.g. for competitive sports, the more thiamine the food must contain. It also prevents the accumulation of toxic by-products of cell metabolism that may damage the heart or nervous system.
Product advantages
- No post-column derivatisation
- Simple handling
- Stable standards and controls
TPP can be determined fast and simply direct from whole blood using this Chromsystems kit. The kit is optimised for isocratic HPLC systems with fluorescence detector. The derivatisation necessary for fluorescence detection is included in the sample preparation procedure, and there is no need for instrument-intensive post-column derivatisation for the fluorescence detection. A special HPLC column and an optimised mobile phase allow precise and reliable quantification in less than 6 minutes.
Method of Analysis | HPLC |
---|---|
Number of Tests | 100 |
Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
Lower Limit of Quantitation | 0.8 μg/l |
Upper Limit of Quantification | up to at least 750 μg/l |
Intraassay | CV = 2.8–4.9 % |
Interassay | CV = 4.3–4.7 % |
Recovery | 91 % |
Specimen | Whole Blood |
Sample Preparation |
|
Run Time | 6 min |
Injection Volume | 50 µl |
Flow Rate | 1 ml/min |
Column Temperature | ambient (~ 25 °C) |
Gradient | Isocratic |
Wavelength | EX 367 nm EM 435 nm |
Additional Info | Every isocratic HPLC system with fluorescence detector is suitable. |
Parameters | Vitamin B1 (TPP, thiamine pyrophosphate) |
-
Whole Blood Calibration Standard Vitamins B1/B2Order no.: 37008Vitamin B1/B2 in Whole Blood - HPLC
-
Whole Blood Calibration Standard Vitamins B1/B2Order no.: 37008Vitamin B1/B2 in Whole Blood - HPLC
Vitamin B1 (TPP, thiamine pyrophosphate)
Clinical relevance
Vitamin B1 (thiamine) plays an important role in deriving energy from carbohydrates and fats. The active form is thiamine pyrophosphate (TPP), a coenzyme for some enzymatic reactions in the carbohydrate metabolism and for the pyruvate decarboxylase. TPP is also significantly involved in the oxidative degradation of glucose. The determination of TPP is more significant than measurements of total thiamine, as by this method only the physiologically active form is taken into account.
Thiamine need is closely associated with caloric intake due to its central role in the energy metabolism: the more energy is needed, e.g. for competitive sports, the more thiamine the food must contain. It also prevents the accumulation of toxic by-products of cell metabolism that may damage the heart or nervous system.
Product advantages
- No post-column derivatisation
- Simple handling
- Stable standards and controls
TPP can be determined fast and simply direct from whole blood using this Chromsystems kit. The kit is optimised for isocratic HPLC systems with fluorescence detector. The derivatisation necessary for fluorescence detection is included in the sample preparation procedure, and there is no need for instrument-intensive post-column derivatisation for the fluorescence detection. A special HPLC column and an optimised mobile phase allow precise and reliable quantification in less than 6 minutes.
Method of Analysis | HPLC |
---|---|
Number of Tests | 100 |
Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
Lower Limit of Quantitation | 0.8 μg/l |
Upper Limit of Quantification | up to at least 750 μg/l |
Intraassay | CV = 2.8–4.9 % |
Interassay | CV = 4.3–4.7 % |
Recovery | 91 % |
Specimen | Whole Blood |
Sample Preparation |
|
Run Time | 6 min |
Injection Volume | 50 µl |
Flow Rate | 1 ml/min |
Column Temperature | ambient (~ 25 °C) |
Gradient | Isocratic |
Wavelength | EX 367 nm EM 435 nm |
Additional Info | Every isocratic HPLC system with fluorescence detector is suitable. |
Parameters | Vitamin B1 (TPP, thiamine pyrophosphate) |
-
Whole Blood Calibration Standard Vitamins B1/B2Order no.: 37008Vitamin B1/B2 in Whole Blood - HPLC
-
Whole Blood Calibration Standard Vitamins B1/B2Order no.: 37008Vitamin B1/B2 in Whole Blood - HPLC